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Consiglio Nazionale delle Ricerche

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Anna Boccaccio^1,2, Claudia Sagheddu¹, Anna Menini^1,3

¹SISSA, International School for Advanced Studies, ²Istituto di Biofisica, Consiglio Nazionale delle Ricerche, ³SISSA Unit, Italian Institute of Technology

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.

Education

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Chryssostomos Chatgilialoglu¹, Carla Ferreri¹, Annalisa Masi¹, Michele Melchiorre¹, Anna Sansone¹, Michael A. Terzidis¹, Armida Torreggiani¹

¹ISOF - Bio Free Radicals, Consiglio Nazionale delle Ricerche

Radical-based biomimetic chemistry has been applied to building-up libraries necessary for biomarker development.