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The University of Tulsa

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Jeanmarie Verchot¹, Aastha Thapa², Dulanjani Wijayasekara³, Peter R. Hoyt⁴

¹Texas A&M Agrilife Center at Dallas, ²Noble Research Center, Oklahoma State University, ³Department of Biology, College of Engineering and Natural Sciences, The University of Tulsa, ⁴Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology, Oklahoma State University

Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.

Environment

Simulation of Early Earth Hydrothermal Chimneys in a Thermal Gradient Environment

Ninos Hermis¹, Gabriel LeBlanc², Laura M. Barge¹

¹NASA Jet Propulsion Laboratory, California Institute of Technology, ²The University of Tulsa

The goal of this protocol is to form simulated hydrothermal chimneys via chemical garden injection experiments and introduce a thermal gradient across the inorganic precipitate membrane, using a 3D printable condenser that can be reproduced for educational purposes.

Genetics

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Amy Smith¹, Mary Bergwell^*1, Ellie Smith^*1, Danita Mathew^*1, Jyoti Iyer¹

¹Department of Chemistry and Biochemistry, The University of Tulsa

The goal of this protocol is to enable seamless CRISPR/Cas9 editing of the C. elegans genome using assembled ribonucleoprotein complexes and the dpy-10 co-CRISPR marker for screening. This protocol can be used to make a variety of genetic modifications in C. elegans including insertions, deletions, gene replacements and codon substitutions.