CRISPR/Cas9 Dpy-10 공동 CRISPR 스크리닝 마커 및 조립된 리보뉴클레오단백질 복합체를 사용하여 C. elegans rbm-3.2 유전자의 편집.

0:53

C. elegans Gonad Preparation and Microinjection

1:56

Injected Worm Recovery and Transfer

2:37

PickingC. elegans for Screening

3:17

Single Worm Lysis and PCR

4:22

Restriction Digestion and Agarose Gel Electrophoresis

5:08

Identification of Positively Edited and Homozygous Edited Worms

6:01

Results: Representative C. elegans rbm-3.2 Gene Editing and Screening

7:10

Conclusion