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Wyss Institute for Biologically Inspired Engineering

2 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

CRISPR Guide RNA Cloning for Mammalian Systems
Sathiji Nageshwaran *1,2, Alejandro Chavez *1,2,3, Nan Cher Yeo 1,2, Xiaoge Guo 1,2, Alissa Lance-Byrne 1, Angela Tung 1, James J. Collins 1,4,5,6,7, George M. Church 1,2
1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute

Here, a simple, efficient, and cost-effective method of sgRNA cloning is outlined.

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Bioengineering

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens
Daniel J. Wiegand 1,2, Henry H. Lee 1, Nili Ostrov *1, George M. Church *1,2
1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering

Cell-free expression systems are powerful and cost-efficient tools for the high-throughput synthesis and screening of important proteins. Here, we describe the preparation of cell-free protein expression system using Vibrio natriegens for the rapid protein production using plasmid DNA, linear DNA, and mRNA template.

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