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Hong Kong Baptist University

5 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Po Ying Poon¹, Patrick Ying Kit Yue¹, Ricky Ngok Shun Wong¹

¹Department of Biology, Hong Kong Baptist University

Here, we present a protocol to perform a semi-high throughput cell migration assay on a 96-well cell culture plate. This protocol is a fast, simple and economical method to create consistent scratch wounds on a cell monolayer.

Behavior

Comparative Analysis of Lower Limb Kinematics between the Initial and Terminal Phase of 5km Treadmill Running

Wenjing Quan¹, Meizi Wang², Gongju Liu², Gusztáv Fekete³, Julien S. Baker⁴, Feng Ren¹, Yaodong Gu¹

¹Faculty of Sports Science, Ningbo University, ²Faculty of Engineering, University of Pannonia Veszeprem, ³Savaria Institute of Technology, Eötvös Loránd University, ⁴Department of Sport, and Physical Education, Hong Kong Baptist University

This study investigated the biomechanical characteristics of the lower extremity kinematic variables between the initial and terminal phase of 5 km treadmill running. The lower-limb kinematic data of 10 runners were collected using a three-dimensional motion capture system on a treadmill at the initial phase (0.5 km) and the terminal phase (5 km), respectively.

JoVE Journal

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor

Jason Cho Wai Lo¹, Wee Lin Wong¹, Catherine Hong Huan Hor¹

¹Department of Chemistry, Faculty of Science, Hong Kong Baptist University

Here, we present a reproducible in vitro electroporation protocol for genetic manipulation of primary cerebellar granule cell precursors (GCPs) that is cost-effective, efficient, and viable. Moreover, this protocol also demonstrates a straightforward method for the molecular study of primary cilium-dependent Hedgehog signaling pathways in primary GCP cells.

JoVE Core

Meta-Analysis of the Effectiveness and Safety of Shugan Jieyu Capsules for the Treatment of Insomnia

Jing Wang^*1, Peipei Du^*2, Linda LD Zhong², Yaxuan Ren¹, Li Feng¹, Shu Yang¹, Haibei Song¹

¹School of Intelligent Medicine, Chengdu University of Traditional Chinese Medicine, ²School of Chinese Medicine, Hong Kong Baptist University

The presented protocol describes an investigation strategy and systematic review to evaluate the clinical effectiveness and safety of Shugan Jieyu capsules for treating insomnia.

Behavior

Integration of Animal Behavioral Assessment and Convolutional Neural Network to Study Wasabi-Alcohol Taste-Smell Interaction

Hiu-Lok Ngan¹, Zenghua Qi², Hong Yan¹, Yuanyuan Song¹, Tao Wang^1,3, Zongwei Cai^1,2

¹State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, ²Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, ³Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, and School of Pharmacy, Guangdong Medical University

This article describes a set of methods for measuring the suppressive ability of sniffing alcoholic beverages on the wasabi-elicited stinging sensation.