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German Centre for integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig

1 ARTICLES PUBLISHED IN JoVE

Chemistry

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Katharina Grosser^1,2, Nicole M. van Dam^1,2

¹German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, ²Institute of Ecology, Friedrich Schiller University Jena

Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.