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Portland State University

2 ARTICLES PUBLISHED IN JoVE

Biology

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

H. Arthur Jeiranian^*1, Brandy J. Schalow^*1, Justin Courcelle¹

¹Department of Biology, Portland State University

We present a procedure by which two-dimensional agarose-gel analysis can be used to identify the structure of replication intermediates that occur following UV irradiation.

Engineering

Use of Sacrificial Nanoparticles to Remove the Effects of Shot-noise in Contact Holes Fabricated by E-beam Lithography

Shankar B. Rananavare¹, Moshood K. Morakinyo²

¹Department of Chemistry, Portland State University, ²Logic Technology Department, Intel Corporation

Uniformly sized nanoparticles can remove fluctuations in contact hole dimensions patterned in poly(methyl methacrylate) (PMMA) photoresist films by electron beam (E-beam) lithography. The process involves electrostatic funneling to center and deposit nanoparticles in contact holes, followed by photoresist reflow and plasma- and wet-etching steps.