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Wroclaw Medical University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Magdalena Zemelka-Wiacek¹, Monika Majewska-Szczepanik², Pawel Gajdanowicz¹, Marian Szczepanik³

¹Department of Clinical Immunology, Wroclaw Medical University, ²Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, ³Chair of Biomedical Sciences, Faculty of Health Sciences, Jagiellonian University Medical College

Contact hypersensitivity (CHS) is a murine experimental model of allergic contact dermatitis (ACD). CHS is based on sensitization with reactive hapten by painting the shaved skin of the chest and abdomen, with a subsequent ear skin challenge with a diluted hapten, causing a swelling reaction that is assessed in various ways.

Immunology and Infection

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Magdalena Zemelka-Wiacek¹, Anna Kosowska^1,2, Marek Jutel^1,2

¹Department of Clinical Immunology, Wroclaw Medical University, ²ALL-MED Medical Research Institute

A protocol for conducting a challenge in an allergen exposure chamber (AEC) facility is presented. AECs have proven to be safe and effective tools for the induction of allergic symptoms or as an endpoint in the efficacy testing of allergen immunotherapy due to their ability to maintain stable particle concentrations and environmental conditions.