Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain AntibodiesK. Martin Chow 1, Sidney W. Whiteheart 1, Jeffrey R. Smiley 2, Savita Sharma 1, Kathy Boaz 2, Meggie J. Coleman 2, Alvina Maynard 3, Louis B. Hersh 1, Craig W. Vander Kooi 1
1Department of Molecular and Cellular Biochemistry, University of Kentucky, 2Division of Laboratory Animal Resources, University of Kentucky, 3River Hill Ranch
A method for the production of single domain antibodies from alpacas, including immunization, blood collection, B-cell isolation, and selection is described.
