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JoVE Journal

Biochemistry

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Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

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05:27 min

January 26th, 2019

January 26th, 2019

22,795 Views

0:04

Title

1:39

Generation of Antigen-specific Alpaca Antibodies

3:02

Purifying Lymphocytes for Single Domain Antibody Library Construction

3:59

Results: Single Domain Antibodies Generated against a Range of Protein Antigens in Alpacas (Lama pacos)

4:46

Conclusion

필기록

A method for the production of single domain antibodies from alpacas, including immunization, blood collection, B-cell isolation, and selection is described.

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