Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel RenaturationBeáta Bozóki *1,2, János András Mótyán *1, Márió Miczi 1, Lívia Diána Gazda 1, József Tőzsér 1
1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, 2Biotechnology Research Department, Gedeon Richter Plc
Here, we present the detailed procedure of a recently developed protease assay platform utilizing N-terminal hexahistidine/maltose-binding protein and fluorescent protein-fused recombinant substrates attached to the surface of nickel-nitrilotriacetic acid magnetic agarose beads. A subsequent in-gel analysis of the assay samples separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is also presented.
