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Taras Shevchenko National University of Kyiv

1 ARTICLES PUBLISHED IN JoVE

Chemistry

In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer Photopharmacology

Kateryna Horbatok^*1,2, Tetyana Makhnii^*2, Viktoriia Kosach², Volodymyr Danko¹, Andrey Kovalenko¹, Stanislav Fatiushchenkov¹, Petro Borysko², Iryna Pishel², Oleg Babii^3,4, Anne S. Ulrich³, Tim Schober⁴, Sergii Afonin³, Igor V. Komarov^1,2,4

¹Taras Shevchenko National University of Kyiv, ²Bienta/Enamine, ³Karlsruhe Institute of Technology, ⁴Lumobiotics

This protocol presents a set of experiments adopted for the evaluation of photoswitchable anticancer peptides, that can be used in the preclinical screening of such compounds. This includes cytotoxicity assessment in 2D and 3D cell cultures, the evaluation of ex vivo (model tissue) photoisomerization efficiency, and in vivo efficacy.