Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human ProductAna Marie Landin 1, Cheryl Cox 1, Bin Yu 2, Nelli Bejanyan 1,2, Marco Davila 1,2, Linda Kelley 1,2
1Cell Therapy Facility, Moffitt Cancer Center and Research Institute, 2Department of Immunology, Moffitt Cancer Center and Research Institute
Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.
