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National Taichung University of Education

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Chien-Wei Cheng¹, Shwu-Yuan Lee², Tang-Yu Chen¹, Jeu-Ming P. Yuann¹, Chi-Ming Chiu¹, Shiuh-Tsuen Huang^3,4, Ji-Yuan Liang¹

¹Department of Biotechnology, Ming Chuan University, ²Department of Tourism and Leisure, Hsing Wu University, ³Department of Science Education and Application, National Taichung University of Education, ⁴Department of Soil and Environmental Sciences, National Chung Hsing University

Here, we present a protocol to inactivate pathogenic bacteria with reactive oxygen species produced during photolysis of flavin mononucleotide (FMN) under blue and violet light irradiation of low intensity. FMN photolysis is demonstrated to be a simple and safe method for sanitary processes.