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Albert Einstein College of Medicine - Yeshiva University

3 ARTICLES PUBLISHED IN JoVE

Biology

Dendra2 Photoswitching through the Mammary Imaging Window

Bojana Gligorijevic^1,2, Dmitriy Kedrin¹, Jeffrey E Segall¹, John Condeelis^1,2, Jacco van Rheenen^1,2,3

¹Department of Anatomy and Structural Biology, Albert Einstein College of Medicine - Yeshiva University, ²Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine - Yeshiva University, ³Hubrecht Institute-KNAW and University Medical Center Utrecht

Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.

Biology

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

Louisiane Perrin¹, Theodore Tucker¹, Bojana Gligorijevic^1,2

¹Bioengineering Department, Temple University, ²Cancer Biology Program, Fox Chase Cancer Center

Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion.

Biology

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Larissa Mourao^1,2, Marta Ciwinska^1,2, Jacco van Rheenen³, Colinda L. G. J. Scheele^1,2

¹Center for Cancer Biology, VIB (BE), ²Department of Oncology, KU Leuven (BE), ³Netherlands Cancer Institute, Department of Molecular Pathology, Oncode Institute (NL)

This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.