This protocol can be used to study the mechanisms governing the invasion of cancer cells into the extracellular matrix in real-time. The main advantage of this technique is that it uses a simple spheroid imaging device, which makes the spheroid in
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Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion.