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Georgia Tech and Emory University

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Douglas Nam¹, Chih-Wen Ni², Amir Rezvan¹, Jin Suo², Klaudia Budzyn¹, Alexander Llanos¹, David G. Harrison¹, Don P. Giddens², Hanjoong Jo^1,2,3

¹Department of Medicine, Division of Cardiology, Emory University, ²Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, ³Department of Bioinspired Science, Ewha Womans University

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

Biology

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Sandeep Kumar^*1, Aitor Andueza^*1, Nicolas Villa-Roel¹, Juyoung Kim¹, Dong-Won Kang¹, Hanjoong Jo^1,2

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²Division of Cardiology, Georgia Institute of Technology and Emory University

We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments.