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Medical College of Georgia

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

Mary Zimmerman^*1, Xiaolin Hu^*1, Kebin Liu¹

¹Department of Biochemistry and Molecular Biology, Medical College of Georgia

An experimental lung metastasis and CTL immunotherapy mouse model for analysis of tumor cells-T cell interaction in vivo.

Biology

Multicolor Time-lapse Imaging of Transgenic Zebrafish: Visualizing Retinal Stem Cells Activated by Targeted Neuronal Cell Ablation

Junko Ariga^*1, Steven L. Walker^*1, Jeff S. Mumm¹

¹Department of Cellular Biology and Anatomy, Medical College of Georgia

In this video, techniques for multicolor confocal time-lapse imaging and targeted cell ablation are provided. Time-lapse imaging is used to monitor the behavior of multiple cell types of interest in vivo. Targeted cell ablation facilitates the study neural circuit function and cell-specific neuronal regeneration paradigms.

Medicine

An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

Amy V. Paschall^1,2,3, Kebin Liu^1,2,3

¹Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, ²Georgia Cancer Center, Augusta University, ³Charlie Norwood VA Medical Center

An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.