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Hadassah-Hebrew University Medical Center

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Felix Moltzahn^1,2,3, Nathan Hunkapiller^1,2,4, Alain A. Mir⁵, Tal Imbar^1,2,6, Robert Blelloch^1,2,3,7

¹The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco , ²Center for Reproductive Sciences, University of California San Francisco , ³Department of Urology, University of California San Francisco , ⁴Department of Cell and Tissue Biology, University of California San Francisco , ⁵Fluidigm Corporation, Fluidigm Corporation , ⁶Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, ⁷UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

Here we describe an optimized multiplex reverse transcriptase quantitative PCR (qRT-PCR) protocol in combination with a microfluidic platform as a cost and time effective high-throughput screening tool for microRNA (miRNA) expression levels, especially when working with limited amounts of sample.

Immunology and Infection

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Ronit Vogt Sionov¹, Simaan Assi¹, Maya Gershkovitz¹, Jitka Y. Sagiv¹, Lola Polyansky¹, Inbal Mishalian², Zvi G. Fridlender², Zvi Granot¹

¹Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, ²Institute of Pulmonary Medicine, Hadassah-Hebrew University Medical Center

Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo.