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Institut für Angewandte Forschung

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)

Michael Wagner¹, Petra Weber¹, Harald Baumann¹, Herbert Schneckenburger¹

¹Hochschule Aalen, Institut für Angewandte Forschung

Topology of cell adhesion on a substrate is measured with nanometre precision by variable-angle total internal reflection fluorescence microscopy (VA-TIRFM).

Engineering

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Thomas Bruns¹, Sarah Schickinger¹, Herbert Schneckenburger¹

¹Institute of Applied Research, Aalen University

A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.

Environment

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Anna Depetris¹, Antoine Wiedmer², Michael Wagner³, Sebastian Schäfer⁴, Tom J. Battin¹, Hannes Peter¹

¹Stream Biofilm and Ecosystem Research Laboratory, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne, ²Plateforme technique, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne, ³Water Chemistry and Water Technology, Engler-Bunte-Institut, Karlsruhe Institute of Technology, ⁴Thorlabs GmbH

Microbial biofilms form complex architectures at interphases and develop into highly scale-dependent spatial patterns. Here, we introduce an experimental system (hard- and software) for the automated acquisition of 3D optical coherence tomography (OCT) datasets. This toolset allows the non-invasive and multi-scale characterization of biofilm morphogenesis in space and time.