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Goethe University Frankfurt

4 ARTICLES PUBLISHED IN JoVE

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Biology

Introduction to Solid Supported Membrane Based Electrophysiology
Andre Bazzone 1, Wagner Steuer Costa 2, Markus Braner 1, Octavian Călinescu 1, Lina Hatahet 1, Klaus Fendler 1
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, 2Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt

Here we present an electrophysiological method based on solid supported membranes with focus on its applications for the characterization of electrogenic membrane transporters.

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Biology

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Michael Urban 1, Marc Vor der Brüggen 2, Robert Tampé 1
1Institute of Biochemistry, Biocenter, Goethe University Frankfurt, 2Nanospot GmbH

The presented protocol describes the analysis of membrane protein mediated transport on the single transporter level using pore-spanning solvent-free lipid bilayers. This is achieved by the creation of bulk produced nanopore array chips, combined with highly parallel data acquisition and analysis, enabling the future establishment of membrane protein effector screenings.

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Biochemistry

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs
Roman Levin 1, Zoe Koeck 1, Volker Dötsch 1, Frank Bernhard 1
1Institute of Biophysical Chemistry, Goethe University Frankfurt

Co-translational insertion into pre-formed nanodiscs makes it possible to study cell-free synthesized membrane proteins in defined lipid environments without contact with detergents. This protocol describes the preparation of essential system components and the critical parameters for improving expression efficiency and sample quality.

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iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology
Hans Wienk 1, Lucia Banci 2, Susan Daenke 3, Eva Pereiro 4, Harald Schwalbe 5, David Ian Stuart 6, Manfred S. Weiss 7, Anastassis Perrakis 1
1Division of Biochemistry and Oncode Institute, The Netherlands Cancer Institute, Amsterdam, 2CERM/CIRMMP, University of Florence, 3Instruct-ERIC, Oxford University, 4MISTRAL beamline, Alba Light Source, 5Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, 6Division of Structural Biology, Oxford University, 7Macromolecular Crystallographyy, Helmholtz-Zentrum Berlin

iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology

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