Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coliLouise E. Bird 1, Heather Rada 1, Anil Verma 1, Raphael Gasper 2,3, James Birch 4, Matthew Jennions 4, Jan Lӧwe 3, Isabel Moraes 4, Raymond J. Owens 1
1Oxford Protein Production Facility, Research Complex at Harwell, 2Protein Crystallography Group, Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory, Diamond Light Source
A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.
