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Memorial University of Newfoundland

3 ARTICLES PUBLISHED IN JoVE

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Behavior

Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test
R. Brian Roome 1,2, Jacqueline L. Vanderluit 1
1BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, 2Neurosciences, Faculty of Medicine, McGill University

Classical forelimb asymmetry analysis of the cylinder test is routinely used to assess behavioural deficits in rats following brain injury or stroke; however, it fails to detect consistent deficits in mice. This study demonstrates that quantifying paw-dragging behaviour is a more sensitive analysis of brain injury in mice.

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Biochemistry

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Ryan J. Mailloux 1, Adrian Young 1, Marisa O'Brien 1, Robert Morris Gill 1
1Department of Biochemistry, Memorial University of Newfoundland

Mitochondria contain several flavin-dependent enzymes that can produce reactive oxygen species (ROS). Monitoring ROS release from individual sites in mitochondria is challenging due to unwanted side reactions. We present an easy, inexpensive method for direct assessment of native rates for ROS release using purified flavoenzymes and microplate fluorometry.

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Environment

Determination of Total Lipid and Lipid Classes in Marine Samples
C. C. Parrish 1, J. S. Wells 1
1Department of Ocean Sciences and CREAIT Network, Memorial University of Newfoundland

This protocol is for the determination of lipids in seawater and biological specimens. Lipids in filtrates are extracted with chloroform or mixtures of chloroform and methanol in the case of solids. Lipid classes are measured by rod thin-layer chromatography with flame ionization detection and their sum gives the total lipid content.

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