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Bundeswehr Institute of Pharmacology and Toxicology

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Dirk Steinritz^1,2, Annette Schmidt^1,3, Frank Balszuweit¹, Horst Thiermann¹, Marwa Ibrahim³, Birgit Bölck³, Wilhelm Bloch³

¹Bundeswehr Institute of Pharmacology and Toxicology, ²Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-Universität München, ³Department of Molecular and Cellular Sports Medicine, German Sports University Cologne

Investigation of early endothelial cell (EEC) migration is important to understand the pathophysiology of certain illnesses and to potentially identify novel strategies for therapeutic intervention. The following protocol describes techniques to assess cell migration that have been adapted for the investigation of EEC.

Medicine

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

Amelie Tsoutsoulopoulos¹, Katrin Gohlsch², Niklas Möhle³, Andreas Breit², Sebastian Hoffmann⁵, Olaf Krischenowski^3,4, Harald Mückter², Thomas Gudermann², Horst Thiermann¹, Michaela Aufderheide^3,4, Dirk Steinritz^1,2

¹Bundeswehr Institute of Pharmacology and Toxicology, ²Walther Straub Institute of Pharmacology and Toxicology, University of Munich, ³Cultex Laboratories GmbH, ⁴Cultex Technologies GmbH (formerly Cultex Laboratories GmbH), ⁵seh consulting + services

We present a robust, transferable and predictive in vitro exposure system for the screening and monitoring of airborne particles concerning their acute pulmonary cytotoxicity by exposing cultivated human lung cells at the air-liquid interface (ALI).