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Korea Institute of Science and Technology

8 ARTICLES PUBLISHED IN JoVE

Neuroscience

Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits

Hye Yun Kim^1,2, Dongkeun K. Lee¹, Bo-Ryehn Chung^1,3, Hyunjin V. Kim^1,4, YoungSoo Kim^1,4

¹Center for Neuro-Medicine, Korea Institute of Science and Technology, ²Research Institute, GoshenBiotech, Inc., ³Department of Chemical and Biological Engineering, Princeton University, ⁴Biological Chemistry Program, Korea University of Science and Technology

The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice.

Neuroscience

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Sungmoo Lee^1,2, Hong Hua Piao², Masoud Sepheri-Rad², Arong Jung^2,3, Uhna Sung², Yoon-Kyu Song^1,4, Bradley J. Baker²

¹Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, ²Center for Functional Connectomics, Korea Institute of Science and Technology, ³College of Life Sciences and Biotechnology, Korea University, ⁴Advanced Institutes of Convergence Technology

A method for imaging changes in membrane potential using genetically encoded voltage indicators is described.

Medicine

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Dong-Eog Kim¹, Jeong-Yeon Kim¹, Su-Kyoung Lee¹, Ju Hee Ryu², Ick Chan kwon², Cheol-Hee Ahn³, Kwangmeyung Kim², Dawid Schellingerhout⁴

¹Molecular Imaging and Neurovascular Research Laboratory, Dongguk University College of Medicine, ²Biomedical Research Center, Korea Institute of Science and Technology, ³Research Institute of Advanced Materials, Department of Materials Science and Engineering, Seoul National University, ⁴Departments of Radiology and Cancer Systems Imaging, University of Texas M.D. Anderson Cancer Center

This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.

Bioengineering

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Minyoung Kim^1,2, Hyunjoon Rhee^1,3, Ji Yoon Kang¹, Tae Song Kim¹, Rhokyun Kwak¹

¹Center for BioMicrosystems, Korea Institute of Science and Technology, ²Department of Mechanical Engineering, Seoul National University, ³Department of Industrial Engineering, University of Illinois Urbana-Champaign

The protocol for a novel ion concentration polarization (ICP) platform that can stop the propagation of the ICP zone, regardless of the operating conditions is described. This unique ability of the platform lies in the use of merging ion depletion and enrichment, which are two polarities of the ICP phenomenon.

Medicine

Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans

So Young Lee¹, Kyungsu Kang¹

¹Systems Biotechnology Research Center, Korea Institute of Science and Technology

A basic protocol to evaluate the toxicity of chemicals in a model animal, Caenorhabditis elegans, is described. The method is convenient and useful for the development of pharmaceuticals as well as for the risk assessment of various environmental pollutants.

Neuroscience

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

Anvar Sariev^1,2, Jinho Chung¹, Dajung Jung^1,3, Farnaz Sharif^1,2, Ju-Young Lee^1,2, Soyoun Kim¹, Sebastien Royer^1,2

¹Center for Functional Connectomics, Korea Institute of Science and Technology, ²Biomedical department, KIST school, University of Science and Technology, ³Department of Biological Sciences, Korea Advanced Institute of Science and Technology

We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.

Bioengineering

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

Youngmin Seo^*1, Chanhwi Park^*2, Jaewoo Son², Kyungwoo Lee², Jangsun Hwang², Yeonho Jo², Dohyun Lee², Muhammad Saad Khan², Sachin Ganpat Chavan², Yonghyun Choi², Dasom Kim², Assaf A Gilad³, Jonghoon Choi²

¹Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, ²School of Integrative Engineering, Chung-Ang University, ³Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University

In this study, antimicrobial nanomaterials were synthesized by acidic oxidation of multiwalled carbon nanotubes and subsequent reductive deposition of silver nanoparticles. Antimicrobial activity and cytotoxicity tests were performed with the as-prepared nanomaterials.

Medicine

Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans

Tram Anh Ngoc Le¹, Baskar Selvaraj^1,2, Jae Wook Lee^1,2, Kyungsu Kang^1,2

¹Gangneung Institute of Natural Products, Korea Institute of Science and Technology, ²Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST)

This protocol describes how to measure intestinal permeability of Caenorhabditis elegans. This method is helpful for basic biological research on intestinal health related to the interaction between intestinal bacteria and their host and for screening to identify probiotic and chemical agents to cure leaky gut syndrome and inflammatory bowel diseases.