Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein ResolutionMichael Urban 1, Marc Vor der Brüggen 2, Robert Tampé 1
1Institute of Biochemistry, Biocenter, Goethe University Frankfurt, 2Nanospot GmbH
The presented protocol describes the analysis of membrane protein mediated transport on the single transporter level using pore-spanning solvent-free lipid bilayers. This is achieved by the creation of bulk produced nanopore array chips, combined with highly parallel data acquisition and analysis, enabling the future establishment of membrane protein effector screenings.
