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Charité - Universitätsmedizin, Berlin

2 ARTICLES PUBLISHED IN JoVE

Medicine

Lavage-induced Surfactant Depletion in Pigs As a Model of the Acute Respiratory Distress Syndrome (ARDS)

Martin Russ¹, Sebastian Kronfeldt¹, Willehad Boemke¹, Thilo Busch², Roland C. E. Francis^*1, Philipp A. Pickerodt^*1

¹Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin, Berlin, ²Department of Anesthesiology and Intensive Care Medicine, University of Leipzig Medical Faculty

Repeated pulmonary lavages in anesthetized pigs induce lung injury resembling major aspects of human acute respiratory distress syndrome (ARDS). For this purpose the lungs are repeatedly lavaged with 0.9% saline at 37 °C. The goal of the protocol is a reproducible mitigation of gas exchange and hemodynamics for research in ARDS.

Medicine

Surfactant Depletion Combined with Injurious Ventilation Results in a Reproducible Model of the Acute Respiratory Distress Syndrome (ARDS)

Martin Russ^*1, Emilia Boerger^*1, Philip von Platen², Roland C. E. Francis¹, Mahdi Taher¹, Willehad Boemke¹, Burkhard Lachmann¹, Steffen Leonhardt², Philipp A. Pickerodt¹

¹Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin and Berlin Institute of Health Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, ²Chair for Medical Information Technology, RWTH Aachen University

A combination of surfactant washout using 0.9% saline (35 mL/kg body weight, 37 °C) and high tidal volume ventilation with low PEEP to cause moderate ventilator induced lung injury (VILI) results in experimental acute respiratory distress syndrome (ARDS). This method provides a model of lung injury with low/limited recruitability to study the effect of various ventilation strategies for extended periods.