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Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Anush Arakelyan¹, Wendy Fitzgerald¹, Sonia Zicari¹, Murad Vagida², Jean-Charles Grivel³, Leonid Margolis¹

¹Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, ²Laboratory of Atherothrombosis, Cardiology Department, Moscow State University of Medicine and Dentistry, ³Sidra Medical and Research Center

Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.

Immunology and Infection

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Andrea Introini^1,2, Christophe Vanpouille², Wendy Fitzgerald², Kristina Broliden¹, Leonid Margolis²

¹Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital, Karolinska Institutet, ²Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health

Infection of human tissues with human immunodeficiency virus (HIV) ex vivo provides a valuable 3D model of virus pathogenesis. Here, we describe a protocol to process and infect tissue specimens from human tonsils and female genital mucosae with HIV-1 and maintain them in culture at the liquid-air interface.