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University Hospital Aachen

2 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Christoph Emontzpohl^1,2, David Simons³, Sandra Kraemer⁴, Andreas Goetzenich⁴, Gernot Marx¹, Jürgen Bernhagen^5,6, Christian Stoppe¹

¹Department of Intensive Care Medicine, University Hospital Aachen, ²Institute of Biochemistry and Molecular Biology, University Hospital Aachen, ³Department of Radiology, German Cancer Research Center, ⁴Department of Thoracic and Cardiovascular Surgery, University Hospital Aachen, ⁵Department of Vascular Biology, Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, ⁶Deutsches Zentrum für Herz-/Kreislaufkrankheiten (DZHK), Munich Heart Alliance

Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.

Neuroscience

Conducting Hyperscanning Experiments with Functional Near-Infrared Spectroscopy

Vanessa Reindl^1,2, Kerstin Konrad^1,2, Christian Gerloff^2,3, Jana A. Kruppa^1,2,4, Laura Bell¹, Wolfgang Scharke¹

¹Child Neuropsychology Section, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Medical Faculty, RWTH Aachen University, ²JARA-Brain Institute II, Molecular Neuroscience and Neuroimaging, RWTH Aachen & Research Centre Juelich, ³Lehrstuhl II für Mathematik, RWTH Aachen University, ⁴Translational Brain Research in Psychiatry and Neurology, Department of Child and Adolescent Psychiatry, Psychosomatics, and Psychotherapy, University Hospital Aachen

The present protocol describes how to conduct fNIRS hyperscanning experiments and analyze brain-to-brain synchrony. Further, we discuss challenges and possible solutions.