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University of Liege

2 ARTICLES PUBLISHED IN JoVE

Medicine

Methodology for Sputum Induction and Laboratory Processing

Julien Guiot^*1, Sophie Demarche^*1,2, Monique Henket¹, Virginie Paulus¹, Sophie Graff¹, Florence Schleich¹, Jean-Louis Corhay¹, Renaud Louis¹, Catherine Moermans¹

¹Department of Respiratory Medicine, CHU Liege, GIGA I3 Research Group, University of Liege, ²Department of Clinical Pharmacy, CIRM (Center for Interdisciplinary Research on Medicines), University of Liege

Here we describe the technique of sputum induction. This protocol also explains the sputum processing to perform a differential cell count and to collect sputum supernatant and cells for further analysis.

Medicine

Nerve Stimulator-guided Injection of Autologous Stem Cells Near the Equine Left Recurrent Laryngeal Nerve

Charlotte Sandersen¹, Justine Ceusters¹, Alexia Fourez¹, Irene Tosi¹, Helene Graide¹, Jean-Philippe Lejeune^1,2, Didier Serteyn^1,2

¹Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, ²Mont-le-Soie Equine Research Centre

Here, we present a protocol to inject autologous muscle-derived stem cells in the proximity to the recurrent laryngeal nerve with the help of an electrical nerve stimulator. This novel technique may become useful for the treatment of equine recurrent laryngeal neuropathy.