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The University of Auckland

5 ARTICLES PUBLISHED IN JoVE

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Medicine

The Measurement and Treatment of Suppression in Amblyopia
Joanna M. Black 1, Robert F. Hess 2, Jeremy R. Cooperstock 3, Long To 3, Benjamin Thompson 1
1Department of Optometry and Vision Science, University of Auckland, 2Department of Ophthalmology, McGill University , 3Centre for Intelligent Machines, McGill University

Amblyopia is a developmental disorder of the visual cortex that is often accompanied by strong suppression of one eye. We present a new technique for measuring and treating interocular suppression in patients with amblyopia that can be deployed using virtual reality goggles or a portable iPod Touch device.

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Biology

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
Jennifer Eom 1, Vaughan Feisst 1, Louis Ranjard 2, Kerry Loomes 1, Tanvi Damani 1, Victoria Jackson-Patel 1,3, Michelle Locke 4,5, Hilary Sheppard 1, Pritika Narayan 1,6, P. Rod Dunbar 1,7
1School of Biological Sciences, The University of Auckland, 2Research School of Biology, The Australian National University, 3Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, 4Department of Plastic Surgery, Counties Manukau District Health Board, 5Department of Surgery, Faculty of Medical and Health Sciences, The University of Auckland, 6Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences, The University of Auckland, 7Maurice Wilkins Centre, The University of Auckland

Traditional methods of assessing adipogenic differentiation are cheap and easy to use, but are not specific to changes in gene expression. We have developed an assay to quantify mesenchymal cell differentiation into mature adipocytes using a lineage specific marker. This assay has diverse applications across basic research and clinical medicine.

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Behavior

Classical Short-Delay Eyeblink Conditioning in One-Year-Old Children
Lucy K. Goodman 1, Nicola S. Anstice 1,2, Suzanne Stevens 3, Benjamin Thompson 1,4, Trecia A. Wouldes 3
1School of Optometry and Vision Science, The University of Auckland, 2Discipline of Optometry, University of Canberra, 3Department of Psychological Medicine, The University of Auckland, 4School of Optometry and Vision Science, University of Waterloo

This protocol describes an eyeblink conditioning paradigm appropriate for experiments with one-year-old infants. Commercial or custom-made equipment can be used to deliver the stimuli, and data collection and analysis should be performed on the video recordings.

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Education

High Throughput Analysis of Liquid Droplet Impacts
Matheu A.J. Broom 1, Geoff R. Willmott 1,2
1The Department of Physics and The MacDiarmid Institute for Advanced Materials and Nanotechnology, The University of Auckland, 2School of Chemical Sciences, The University of Auckland

This protocol enables efficient collection of experimental high-speed images of liquid drop impacts, and speedy analysis of those data in batches. To streamline these processes, the method describes how to calibrate and set up apparatus, generate an appropriate data structure, and deploy an image analysis script.

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Chemistry

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications
Mahsa - Motshakeri 1, Anthony R. J. Phillips 2,3, Paul A. Kilmartin 1
1School of Chemical Sciences, The University of Auckland, 2School of Biological Sciences, The University of Auckland, 3Surgical and Translational Research Center, The University of Auckland

We describe aqueous and organic solvent systems for the electropolymerization of poly(3,4-ethylenedioxythiophene) to create thin layers on the surface of gold microelectrodes, which are used for sensing low molecular weight analytes.

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