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German Center for Lung Research (DZL)

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration

Jonathan M. Tsai^1,2, Irving L Weissman^1,2, Yuval Rinkevich^3,4

¹Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, ²Department of Developmental Biology, Stanford University School of Medicine, ³Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum München, ⁴German Center for Lung Research (DZL)

Here, we present a new method for partial resection of the left hepatic lobe in neonatal (day 0) mice. This new protocol is suitable for studying acute liver injury and injury response in the neonatal setting.

Immunology and Infection

Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples

Dario L. Frey^*1,2, Matteo Guerra^*1,2,3,4, Marcus A. Mall^1,2,5,6,7, Carsten Schultz^1,3,8

¹Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), ²Dept. of Translational Pulmonology, University of Heidelberg, ³Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, ⁴Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, ⁵Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, ⁶Berlin Institute of Health (BIH), ⁷German Center for Lung Research (DZL), Associated Partner Site, Berlin, ⁸Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University

The protocols herein described provide a guide to visualize and quantify the activity of neutrophil proteases in human sputum. The applications of such analysis span from the evaluation of anti-inflammatory treatments, to biomarker validation, drug screening and large cohort clinical studies.

Biology

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay

Pushkar Ramesh¹, Haifeng Ye¹, Bikram Dasgupta¹, Hans-Günther Machens², Yuval Rinkevich¹

¹Institute of Regenerative Biology and Medicine, Helmholtz Zentrum München, ²School of Medicine, Klinikum Rechts der Isar, Department of Plastic and Hand Surgery, Technical University of Munich

This protocol describes the generation of a skin-fascia explant termed "SCar like tissue in A Dish" or SCAD. This model allows unprecedented visualization of single fibroblasts during scar formation.