Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal CordKaren Haenraets *1,2, Gioele W. Albisetti *1, Edmund Foster 1, Hendrik Wildner 1
1Institute of Pharmacology and Toxicology, University of Zurich, 2Institute of Pharmaceutical Sciences, Swiss Federal Institute (ETH) Zurich
Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.
