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Centro de Investigación Príncipe Felipe

2 ARTICLES PUBLISHED IN JoVE

Biology

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

¹School of Medicine and Dentistry, Universidad Católica de Valencia San Vicente Mártir, ²Unidad Mixta CIPF-UCV, Centro de Investigación Príncipe Felipe, ³Department of Medicine, University of Miami

The goal of the protocol is to show longitudinal intravital real-time tracking of thymocytes by laser scanning microscopy in thymic implants in the anterior chamber of the mouse eye. The transparency of the cornea and vascularization of the graft allows for continuously recording progenitor cell recruitment and mature T-cell egress.

Neuroscience

A Scalable Method to Study Neuronal Survival in Primary Neuronal Culture with Single-cell and Real-Time Resolution

Ángela Rodríguez-Prieto^*1, Ana González-Manteiga^*1, Yaiza Domínguez-Canterla¹, Carmen Navarro-González¹, Pietro Fazzari¹

¹Laboratory of Cortical Circuits in Health and Disease, Centro de Investigación Príncipe Felipe

Here, we present a simple, potent, and versatile methodology to investigate neuronal survival upon cytotoxic stress in primary cortical neurons with cellular resolution in real time or in fixed material.