Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.Kanniah Rajasekaran 1, Ronald J. Sayler 2, Rajtilak Majumdar 1, Christine M. Sickler 1, Jeffrey W. Cary 1
1Southern Regional Research Center, USDA-ARS, Food and Feed Safety Research Unit, 2Department of Plant Pathology, University of Arkansas
Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein. Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.
