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Ochsner Clinic Foundation

5 ARTICLES PUBLISHED IN JoVE

Biology

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Xin Zhang^*1, Keshava Mysore^*2,3, Ellen Flannery^3,4, Kristin Michel¹, David W. Severson^3,4, Kun Yan Zhu⁵, Molly Duman-Scheel^2,3,4

¹Division of Biology, Kansas State University, ²Department of Medical and Molecular Genetics, Indiana University School of Medicine, ³Eck Institute for Global Health, University of Notre Dame, ⁴Department of Biological Sciences, University of Notre Dame, ⁵Department of Entomology, Kansas State University

Here we describe a procedure for inhibiting gene function in disease vector mosquitoes through the use of chitosan/interfering RNA nanoparticles that are ingested by larvae.

JoVE Core

Stress Distribution During Cold Compression of Rocks and Mineral Aggregates Using Synchrotron-based X-Ray Diffraction

Cecilia S.N. Cheung^1,2, Donald J. Weidner¹, Li Li¹, Philip G. Meredith³, Haiyan Chen¹, Matthew Whitaker¹, Xianyin Chen⁴

¹Mineral Physics Institute, Department of Geoscience, Stony Brook University, ²Geological Engineering, Department of Civil and Environmental Engineering, University of Wisconsin-Madison, ³Rock and Ice Physics Laboratory, Department of Earth Sciences, University College London, ⁴Department of Chemistry, Stony Brook University

We report detailed procedures for compression experiments on rocks and mineral aggregates within a multi-anvil deformation apparatus coupled with synchrotron X-radiation. Such experiments allow quantification of the stress distribution within samples, that ultimately sheds light on compaction processes in geomaterials.

Cancer Research

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis

Ravan Moret¹, Linh Hellmers¹, Xin Zhang¹, Jessie Gills², Nathan Hite³, Aaron Klinger³, Grace A. Maresh¹, Daniel Canter^2,4, Stephen Bardot^2,4, David A Margolin^3,4, Li Li^1,4

¹Laboratory of Translational Cancer Research, Institute for Translational Research, Ochsner Clinic Foundation, ²Department of Urology, Ochsner Clinic Foundation, ³Department of Colon and Rectal Surgery, Ochsner Clinic Foundation, ⁴Ochsner Clinical School, University of Queensland, School of Medicine

This protocol describes the generation of patient-derived orthotopic xenograft models by intra-vesically instilling high-grade urothelial cell carcinoma cells or intra-rectally injecting colorectal cancer cells into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice for primary tumor growth and spontaneous metastases under the influence of lymph node stromal cells, which mimics the progression of human metastatic diseases.

Developmental Biology

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Li Li¹, Anna Y. Zinovyeva¹

¹Division of Biology, Kansas State University

This method describes a protocol for high-throughput protein extract preparation from Caenorhabditis elegans samples and subsequent co-immunoprecipitation.

Behavior

Physical Activity Measurement in Children Accepting Table Tennis Training

Xin Zhang^*1, Cuiqiao Xia^*2, Xiuli Zhao³, Yu Liu³, Hóngyi Zhào^2,3, Yonghua Huang²

¹Department of Sport, Inner Mongolia Medical University, ²Department of Neurology, The Seventh Medical Center of PLA General Hospital, ³Department of Neurology, NO 984 Hospital of PLA

This study proposes an accelerometer-based method to objectively measure physical activity (PA) and leisure time physical activity (LTPA) in Chinese children accepting table tennis training in clubs.