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Max Planck Institute of Biology of Ageing

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Mohamed Salem^1,2, Michal Bernach¹, Krzysztof Bajdzienko¹, Patrick Giavalisco¹

¹Max Planck Institute of Molecular Plant Physiology, Golm, Germany, ²Department of Pharmacognosy, Faculty of Pharmacy, Cairo University

A protocol for comprehensive extraction of lipids, metabolites and proteins from biological tissues using one sample is presented.

Developmental Biology

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii

Umarah Mubeen¹, Lais Albuquerque Giraldi¹, Jessica Jüppner¹, Patrick Giavalisco^1,2

¹Max Planck Institute of Molecular Plant Physiology, ²Max Planck Institute of Biology of Ageing

System-wide analysis of multiple biomolecules is crucial to gain functional and mechanistic insights into biological processes. Hereby, an extensive protocol is described for high throughput extraction of lipids, metabolites, proteins and starch from a single sample harvested from synchronized Chlamydomonas culture.