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University of Sonora

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies

María Dolores Figueroa-Pizano¹, Itziar Vélaz², María Elisa Martínez-Barbosa¹

¹Department of Polymers and Materials Research, University of Sonora, ²Department of Chemistry, Faculty of Sciences, University of Navarra

The freezing-thawing method is used to produce chitosan-poly(vinyl alcohol) hydrogels without crosslinking agents. For this method, it is important to consider the freezing conditions (temperature, number of cycles) and polymer ratio, which can affect the properties and applications of the obtained hydrogels.

Neuroscience

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Irina Sinakevitch^1,2, Zev Kurtzman^*2, Hyun G. Choi^*2, David Arturo Ruiz Pardo³, Romain A. Dahan², Nathaniel Klein¹, Branimir Bugarija⁴, Erik Wendlandt⁴, Brian H. Smith²

¹Department of Neuroscience, University of Arizona, ²School of Life Sciences, Arizona State University, ³Department of Scientific and Technologic Investigations, University of Sonora, ⁴Integrated DNA Technologies, Inc.

Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity.