Mercy Health Hauenstein Neuroscience Medical Center
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo
Joseph R. Patterson 1, Nicole K. Polinski 2, Megan F. Duffy 1,3, Christopher J. Kemp 1, Kelvin C. Luk 4, Laura A. Volpicelli-Daley 5, Nicholas M. Kanaan 1, Caryl E. Sortwell 1,3,6
1Department of Translational Science and Molecular Medicine, Michigan State University, 2The Michael J. Fox Foundation for Parkinson's Research, 3Neuroscience Program, Michigan State University, 4Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, 5Center for Neurodegeneration and Experimental Therapeutics, University of Alabama at Birmingham, 6Mercy Health Hauenstein Neuroscience Medical Center
The goal of this article is to outline the steps required for the generation of fibrils from monomeric alpha-synuclein, subsequent quality control, and use of the preformed fibrils in vivo.
Genetics
Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
Joseph R. Patterson 1,2,3, Allyson Cole-Strauss 1,2,3, Nathan Kuhn 1,2, Carlene Mercier 3,4, Joseph Kochmanski 1,2, John A. Gerlach 5, Rhiannon M. LeVeque 3,4, Kerri A. Neugebauer 3,4,6, Kayla N. Conner 3,4, Jasper Gomez 3,4, Mark G. Hennes 3,4, Kaje’ne E. Thompson 3,4, Destinee L. Rytlewski 3,4, Chloe C. Bigwood 3,4, Amy Scharmen 3,4, Gabriel Simjanovski 3,4, Cassidy Riley 3,4, Jessica Donaldson 3,4, Dilann Yasin 3,4, Najwa Kouja 3,5, Zaria Contejean 3,4, Michaela Burnett 3,4, Shakhlo Aminova 3,4, Nat Ato Yawson 3,4,7, Simran B. Singh 3,4, Osama M. Alian 3,4, Carson D. Broeker 3,4,8, Erin K. Zaluzec 3,4,7, Morgan ONeill 3,4,9, Birgit Puschner 10, Aron Sousa 2, Laura Bix 11, Brian Jespersen 2, Claudia Holzman 12, Jade Mitchell 13, Ryan Julien 13, Yesim Askin 14, Danielle Barnes 14, Purna Durshanpalli 14, Doug Krum 14, Rett Weber 14, Morgan Patterson 14, Becky Anderson 14, Ryan Hunt 14, Benjamin O’Brien 14, Andrew Umstead 1,2, John S. Beck 1,2, Irving E. Vega 1,2, Caryl E. Sortwell 1,2, Jack W. Lipton 1,2
1Department of Translational Neuroscience, Michigan State University, 2College of Human Medicine, Michigan State University, 3COVID-19 Early Detection Program, Michigan State University, 4Department of Microbiology and Molecular Genetics, Michigan State University, 5Biomedical Laboratory Diagnostics Program, Michigan State University, 6Department of Plant, Soil, and Microbial Sciences, Michigan State University, 7Department of Pharmacology and Toxicology, Michigan State University, 8Department of Biochemistry and Molecular Biology, Michigan State University, 9College of Osteopathic Medicine, Michigan State University, 10College of Veterinary Medicine, Michigan State University, 11School of Packaging, Michigan State University, 12Department of Epidemiology and Biostatistics, Michigan State University, 13Department of Biosystems Engineering, Michigan State University, 14Enterprise Services, Michigan State University
The protocol is intended to serve as a blueprint for universities and other organizations considering large-scale testing for SARS-CoV-2 or developing preparedness plans for future viral outbreaks.
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