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The Ohio State University Comprehensive Cancer Center

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

Elliott J. Hagedorn^1,2, Jennifer L. Cillis³, Caitlyn R. Curley³, Taylor C. Patch³, Brian Li^1,2, Bradley W. Blaser^1,2,7, Raquel Riquelme^1,2, Leonard I. Zon^1,2,4,5,6, Dhvanit I. Shah^1,2,3,4,5

¹Division of Hematology/Oncology, Boston Children’s Hospital, ²Harvard Medical School, ³Division of Hematology, Department of Medicine, Brigham and Women’s Hospital, ⁴Harvard Stem Cell Institute, ⁵Broad Institute of Massachusetts Institute of Technology, ⁶Howard Hughes Medical Institute, ⁷Division of Hematologic Malignancies, Dana-Farber Cancer Institute

This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.

JoVE Journal

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Giorgia Scapin^1,2,3, Marie C. Goulard^1,2,3, Priyanka R. Dharampuriya^1,2,3, Jennifer L. Cillis^1,2,3, Dhvanit I. Shah^1,2,3

¹Nationwide Children's Hospital, ²The Ohio State University College of Medicine, ³The Ohio State University Comprehensive Cancer Center

Hematopoietic stem progenitor cells (HSPCs) transition from a quiescent state to a differentiation state due to their metabolic plasticity during blood formation. Here, we present an optimized method for measuring mitochondrial respiration and glycolysis of HSPCs.

Medicine

Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography

Qiang Zeng¹, Doug A. Gouchoe^2,3, Mahboubeh Nabavinia⁴, Yong Gyu Lee², Xi Wang², Terri A. Shaffer⁵, Mitchel R. Stacy^4,5,6, Blake R. Peterson^7,8, Bryan A. Whitson^2,9, Christopher Breuer^1,4, Sylvester M. Black^1,10

¹Department of Surgery, Nationwide Children’s Hospital, ²COPPER Laboratory, The Ohio State University, ³88th Surgical Operation Operations Squadron, WPAFB, ⁴Center for Regenerative Medicine, The Research Institute at Nationwide Children’s Hospital, ⁵Division of Vascular Diseases and Surgery, The Ohio State University College of Medicine, ⁶Interdisciplinary Biophysics Graduate Program, The Ohio State University, ⁷Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University College of Pharmacy, ⁸The Ohio State University Comprehensive Cancer Center, ⁹Division of Cardiac Surgery, Department of Surgery, The Ohio State Wexner Medical Center, ¹⁰Division of Transplantation, Department of Surgery, The Ohio State Wexner Medical Center

In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.