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The Joint Unit of the Chinese Academy of Medical Sciences

6 ARTICLES PUBLISHED IN JoVE

Bioengineering

Engineering Platform and Experimental Protocol for Design and Evaluation of a Neurally-controlled Powered Transfemoral Prosthesis

Fan Zhang¹, Ming Liu¹, Stephen Harper^2,3, Michael Lee³, He Huang¹

¹Joint Department of Biomedical Engineering, North Carolina State University & University of North Carolina at Chapel Hill, ²Department of Physical Medicine and Rehabilitation, University of North Carolina School of Medicine, ³Atlantic Prosthetics & Orthotics, LLC

Neural-machine interfaces (NMI) have been developed to identify the user's locomotion mode. These NMIs are potentially useful for neural control of powered artificial legs, but have not been fully demonstrated. This paper presented (1) our designed engineering platform for easy implementation and development of neural control for powered lower limb prostheses and (2) an experimental setup and protocol in a laboratory environment to evaluate neurally-controlled artificial legs on patients with lower limb amputations safely and efficiently.

Medicine

Photoselective Vaporesection of the Prostate via an End-firing Lithium Triborate Crystal Laser

Xin Wang ¹, Yao-Guang Zhang¹, Sheng-Cai Zhu¹, Ben Wan¹, Ming Liu¹, Jian-Ye Wang¹

¹Department of Urology, Beijing Hospital, National Center of Gerontology

Here we present a protocol to perform Photoselective Vaporesection of the Prostate (PVRP) for benign prostatic hyperplasia (BPH) treatment.

JoVE Journal

Determining Tribocorrosion Rate and Wear-Corrosion Synergy of Bulk and Thin Film Aluminum Alloys

Jia Chen^1,2, Hesham Mraied³, Wenjun Cai^1,2

¹Department of Mechanical Engineering, University of South Florida, ²Department of Materials Science and Engineering, Virginia Polytechnic Institute and State University, ³Department of Civil and Environmental Engineering, University of South Florida

Here, we present a protocol to measure the tribocorrosion rate and wear-corrosion synergy of thin film and bulk Al alloys in simulated sea water at room temperature.

Medicine

Invasive Hemodynamic Assessment for the Right Ventricular System and Hypoxia-Induced Pulmonary Arterial Hypertension in Mice

Fuliang Luo¹, Xin Wang¹, Xiaokang Luo¹, Bo Li¹, Desheng Zhu², Hansong Sun³, Yue Tang¹

¹Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Centre for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, ²Laboratory Animal Center, Peking University, ³Center of Cardiac Surgery for Adults, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Centre for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

Here, we present a protocol to perform an invasive hemodynamic assessment of the right ventricle and pulmonary artery in mice using an open-chest surgery approach.

Medicine

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

Chunlong Fu^*1,2, Yaqun Zhang^*1, Xin Wang¹, Shengcai Zhu¹, Huimin Hou¹, Shengjie Liu¹, Cheng Pang¹, Xinda Song¹, Jia Chen¹, Jianye Wang¹, Ming Liu^1,2

¹(Urology) Beijing Hospital, National Center of Gerontology, The Joint Unit of the Chinese Academy of Medical Sciences, ²Graduate School of Peking Union Medical College, Beijing Hospital

Here, we present a protocol to increase the surgical field of view and reduce the difficulty of total transperitoneal laparoscopic nephroureterectomy surgery by precutting the umbilical ligament before treating the terminal ureter.

Neuroscience

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

Sue Han^*1, Xin Wang^*1, Nicholas Cordero¹, Ling-Gang Wu¹

¹National Institute of Neurological Disorders and Stroke

This protocol describes a confocal imaging technique to detect three fusion modes in bovine adrenal chromaffin cells. These fusion modes include 1) close-fusion (also called kiss-and-run), involving fusion pore opening and closure, 2) stay-fusion, involving fusion pore opening and maintaining the opened pore, and 3) shrink-fusion, involving fused vesicle shrinkage.