Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant TissuesMário Costa 1,2, Ana Marta Pereira 3, Sílvia Coimbra 1,2
1Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze, Universitá degli Studi di Milano
This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.
