FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.Hanna Manko 1, Vincent Normant 2,3, Quentin Perraud 2,3, Tania Steffan 1, Véronique Gasser 2,3, Emmanuel Boutant 1, Éléonore Réal 1, Isabelle J. Schalk 2,3, Yves Mély 1, Julien Godet 1,4
1Université de Strasbourg, Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021, 2Université de Strasbourg, UMR 7242, ESBS, 3CNRS, UMR 7242, ESBS, 4Groupe Méthode Recherche Clinique, Hôpitaux Universitaires de Strasbourg
We describe here a protocol to characterize protein-protein interactions between two highly-differently expressed proteins in live Pseudomonas aeruginosa using FLIM-FRET measurements. The protocol includes bacteria strain constructions, bacteria immobilization, imaging and post-imaging data analysis routines.
