Genetic Variant Detection in the CALR gene using High Resolution Melting AnalysisTadej Pajič 1,2, Tanja Belčič Mikič 1, Helena Podgornik 1,3, Jurka Klun 1, Sandra Šućurović 1, Samo Zver 1, Matjaz Sever 1
1Department of Hematology, University Medical Centre Ljubljana, 2Clinical Institute for Genomic Medicine, University Medical Centre Ljubljana, 3Faculty of Pharmacy, University of Ljubljana
High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.
