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University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Irati Romero-Garmendia^*1, Amaia Jauregi-Miguel^*1, Izortze Santin², Jose Ramón Bilbao¹, Ainara Castellanos-Rubio¹

¹Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, ²Endocrinology and Diabetes Research Group, Biocruces Health Research Institute, UPV-EHU, CIBERDEM

Here, we present a protocol to perform a simple cellular fractionation for the subcellular separation of cytoplasmic and nuclear proteins in human fresh and frozen intestinal biopsies.

Cancer Research

A 3D Spheroid Model for Glioblastoma

Joris Guyon^1,2, Laetitia Andrique^1,2, Nadège Pujol^1,2, Gro Vatne Røsland^3,4, Gaelle Recher^1,5,6, Andreas Bikfalvi^1,2, Thomas Daubon^1,2,4

¹University Bordeaux, LAMC, ²INSERM U1029, University Bordeaux, ³Department of Biomedicine, University of Bergen, ⁴CNRS, IBGC UMR5095, ⁵LP2N, CNRS UMR 5298, IOA, ⁶Institut d'Optique Graduate School, IOA

Here, we describe an easy-to-use invasion assay for glioblastoma. This assay is suitable for glioblastoma stem-like cells. A Fiji macro for easy quantification of invasion, migration, and proliferation is also described.

Cancer Research

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Joris Guyon¹, Pierre-Olivier Strale^2,3, Irati Romero-Garmendia⁴, Andreas Bikfalvi¹, Vincent Studer^*2, Thomas Daubon^*4

¹University Bordeaux, INSERM, LAMC, U1029, 33600, Pessac, France, ²Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, Bordeaux, France, ³Alvéole, Bordeaux, France, ⁴University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France

Here, we present an easy-to-use co-culture assay to analyze glioblastoma (GBM) migration on patterned neurons. We developed a macro in FiJi software for easy quantification of GBM cell migration on neurons, and observed that neurons modify GBM cell invasive capacity.