US Army Combat Capabilities Development Command Chemical Biological Center
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Rapid Characterization of Genetic Parts with Cell-Free Systems
John B. McManus 1,2, Casey B. Bernhards 3,4, Caitlin E. Sharpes 3,4, David C. Garcia 2,4, Stephanie D. Cole 4, Richard M. Murray 2, Peter A. Emanuel 4, Matthew W. Lux 4
1US Army Combat Capabilities Development Command Army Research Laboratory, 2Biology and Biological Engineering, California Institute of Technology, 3Excet, Inc., 4US Army Combat Capabilities Development Command Chemical Biological Center
Well-characterized genetic parts are necessary for the design of novel genetic circuits. Here we describe a cost-effective, high-throughput method for rapidly characterizing genetic parts. Our method reduces cost and time by combining cell-free lysates, linear DNA to avoid cloning, and acoustic liquid handling to increase throughput and reduce reaction volumes.
Cell-Free Expression Systems: Methods of Production and Use
Matthew W. Lux 1, Jorge L. Chavez 2, Scott A. Walper 3
1US Army Combat Capabilities Development Command Chemical Biological Center, 2711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson Air Force Base, 3Office of Naval Research – Global
Cell-Free Expression Systems: Methods of Production and Use
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