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The First Affiliated Hospital of Xian Jiao tong University

7 ARTICLES PUBLISHED IN JoVE

Medicine

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Min Jia¹, Fei Meng², Stanley E. Smerin¹, Guoqiang Xing¹, Lei Zhang¹, David M. Su², David Benedek¹, Robert Ursano¹, Yan A. Su², He Li¹

¹Department of Psychiatry, Center for the Study of Traumatic Stress, Uniformed Services University of the Health Sciences, Bethesda, Maryland, ²Department of Gene and Protein Biomarkers, GenProMarkers, Inc.

We describe a rat model of post traumatic stress disorder (PTSD) that reveals the persistent alterations in neuroendocrine function and the delayed long-term, exaggerated fear response, characteristic of PTSD patients. The animal model and methods described here are useful for correlating biomarkers in brain nuclei, which are mechanistic but cannot be measured in patients, with biomarkers in peripheral white blood cells, which can.

Chemistry

Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering

Youngkyu Han¹, Suk-kyun Ahn^2,3, Zhe Zhang^1,4, Gregory S. Smith¹, Changwoo Do¹

¹Biology and Soft Matter Division, Neutron Science Directorate, Oak Ridge National Laboratory, ²Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, ³Department of Polymer Science and Engineering, Pusan National University, ⁴Jülich Center for Neutron Science, Forschungszentrum Jülich

A method for the functionalization of carbon nanotubes with structure-tunable polymeric encapsulation layers and structural characterization using small-angle neutron scattering is presented.

Medicine

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia

Travis B. Nielsen¹, Jun Yan¹, Brian Luna¹, Brad Spellberg^1,2

¹Department of Molecular Microbiology and Immunology, University of Southern California, ²Division of Infectious Diseases, Department of Medicine, LAC+USC Medical Center

Infectious pneumonia is among the most common infections in human. An appropriate in vivo model is critical for understanding disease pathogenesis and testing the efficacy of novel therapeutics. With this murine oropharyngeal aspiration pneumonia model, one can examine the pathogenesis and new treatments against these deadly infections.

Medicine

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch

Yaguang Li¹, Junhong Liu^2,3, Gaixia Liu¹, Zhenhong Pan^2,3, Mingxia Zhang¹, Yao Ma⁴, Qingxia Wei^1,5, Hongping Xia^1,6, Rui Xue Zhang^1,2, Junjun She¹

¹Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, ²Institute of Medical Research, Northwestern Polytechnical University, ³School of Life Sciences, Northwestern Polytechnical University, ⁴Department of Medicine, Xi'an Jiaotong University, ⁵Princess Margaret Cancer Center, University of Health Network, ⁶Department of Pathology, Nanjing Medical University

The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease.

Neuroscience

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

Nannan Zhang¹, Zhe Zhang^1,2, Ilker Ozden², Shinghua Ding^1,2

¹Dalton Cardiovascular Research Center, University of Missouri-Columbia, ²Department of Biomedical, Biological and Chemical Engineering, University of Missouri-Columbia

This proctocol aims to provide a method for in vitro and in vivo mitochondrial Ca²⁺ imaging in astrocytes and neurons.

Medicine

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer

Chenhao Hu^*1,2,3, Zhe Zhang^*1,2,3, Lei Zhang^1,2,3, Ruihan Liu^2,3, Jun Yan^1,2,3, Qi Sun^1,2,3, Guanghui Wang¹, Junjun She^1,2,3

¹Department of General Surgery, The First Affiliated Hospital of Xian Jiaotong University, ²Center for Gut Microbiome Research, Med-X Institute, The First Affiliated Hospital of Xian Jiao tong University, ³Department of High Talent, The First Affiliated Hospital of Xian Jiaotong University

The robotic technique described herein aims to detail a stepwise approach to robot-assisted total mesorectal excision and lateral pelvic lymph node dissection for locally advanced (T3/T4) rectal cancer located below the peritoneal reflection.

Developmental Biology

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Yang Zhang^*1,2,3, Zhe Zhang^*1,2,3, Nannan Kang^1,2,3, Xiaoqiang Sheng^1,2,3

¹Center for Reproductive Medicine and Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, ²State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ³Center for Molecular Reproductive Medicine, Nanjing University

Here, we describe the method of generating an artificial decidualization model using the ovariectomized mouse, a classic endometrial decidualization experiment in the research field of endometrial decidualization.