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the Sixth Affiliated Hospital of Guangzhou Medical University

3 ARTICLES PUBLISHED IN JoVE

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Education

Facile Preparation of (2Z,4E)-Dienamides by the Olefination of Electron-deficient Alkenes with Allyl Acetate
Liyuan Ding 1, Chunbing Yu 1, Zhenqiang Zhao 2, Feifei Li 1, Jian Zhang 1, Guofu Zhong 1
1College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, 2Shijiazhuang Mechanized Infantry Academy

The ruthenium-catalyzed olefination of electron-deficient alkenes with allyl acetate is described here. By using aminocarbonyl as a directing group, this external oxidant-free protocol has high efficiency and good stereo- and regioselectivity, opening a novel synthetic route to (Z,E)-butadiene skeletons.

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Chemistry

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery
Yang Wu *1, Wei Zhang *2, Jian Zhang 2, Zhi-Xiang Mao 3, Li Ding 2, Hao Li 3, Rong Ma 1, Jin-Hai Tang 2
1Research Center of Clinical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Nanjing Medical University Affiliated Cancer Hospital, 2Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, 3School of Clinical Medicine, Xuzhou Medical University

This work presents the preparation of methionine functionalized biocompatible block copolymers (mBG) via the reversible addition-fragmentation chain transfer (RAFT) method. The plasmid DNA complexing ability of the obtained mBG and their transfection efficiency were also investigated. The RAFT method is very beneficial for polymerizing monomers containing special functional groups.

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Medicine

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat
Limei Wang 1, Jianmin Guo 1, Xiaoman Zhong 1, Jian Zhang 2, Yali Sheng 1, Qiwen Lai 1, Hui Song 1, Wei Yang 1
1Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, TCM Non-clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Engineering Research Center for Innovative Drug Evaluation and Research, 2Department of Biomedical Engineering, School of Basic Medical Sciences, the Sixth Affiliated Hospital of Guangzhou Medical University

The present protocol describes a simple and efficient method for collecting blood from the subclavian vein in rats. It enables rapid, timely, and easily identifiable sampling without anesthesia and obtains high-quality blood through repetitive sample collection.

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