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Cambridge University

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
Emily Read *1,2, Geraldine M. Jowett *1,2,3, Diana Coman 1, Joana F. Neves 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University

This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid cells.

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Developmental Biology

A Typical Workflow to Simulate Cytoskeletal Systems
Carlos A. Lugo *1, Eashan Saikia *1, Francois Nedelec 1
1Sainsbury Laboratory, Cambridge University

This protocol demonstrates how to use Cytosim, an Open Source cytoskeleton simulation, to investigate the behavior of a network of filaments connected by molecular motors and passive crosslinkers. A generic workflow with step-by-step instructions is followed to vary the number of crosslinkers and plot the resulting network contractility.

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