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Cambridge University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Emily Read^*1,2, Geraldine M. Jowett^*1,2,3, Diana Coman¹, Joana F. Neves¹

¹Centre for Host-Microbiome Interactions, King’s College London, ²Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, ³Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University

This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid cells.

Developmental Biology

A Typical Workflow to Simulate Cytoskeletal Systems

Carlos A. Lugo^*1, Eashan Saikia^*1, Francois Nedelec¹

¹Sainsbury Laboratory, Cambridge University

This protocol demonstrates how to use Cytosim, an Open Source cytoskeleton simulation, to investigate the behavior of a network of filaments connected by molecular motors and passive crosslinkers. A generic workflow with step-by-step instructions is followed to vary the number of crosslinkers and plot the resulting network contractility.