Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid ModelHeather Liebe 1, Camille Schlegel 2, Xue Cai 2, Alena Golubkova 1, Tyler Leiva 1, William L. Berry 3, Catherine J. Hunter 1
1Division of Pediatric Surgery, Oklahoma Children's Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery, University of Oklahoma Health Sciences Center
The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.
